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    Phylogenetics and dating with

    Karsts represent hotspots of diversity and endemism, but are under-represented in protected areas and under significant threat.

    With many species only occurring on a single site, the lack of protection and research means that the loss of each karst represents an incalculable loss of biodiversity.

    We also perform a time calibration analysis to estimate the age of the clade.

    We find support for the monophyly of Asterophryinae as well as need for taxonomic reclassification of several genera.

    With recent advances in Bayesian clock dating methodology and the explosive accumulation of genetic sequence data, molecular clock dating has found widespread applications, from tracking virus pandemics and studying the macroevolutionary process of speciation and extinction to estimating a timescale for life on Earth.

    Asterophryinae is a large monophyletic subfamily of Anurans containing over 300 species distributed across one of the world's most geologically active areas – New Guinea and its satellite islands, Australia and the Philippines.

    Here we reconstruct a phylogeny for Asterophryinae with greatly increased taxon and genetic sampling relative to prior studies.

    We conducted phylogenetic estimations for 237 cycad species using three genes and two calibration strategies incorporating up to six fossil constraints to Using Bayes factors, we compared divergence time estimates and the inferred dynamics of diversification when using Yule versus birth-death priors.Bayes factors were calculated with marginal likelihood estimated with stepping-stone sampling.We found striking differences in age estimates and diversification dynamics depending on prior choice.The world’s herbaria and other natural history collections house specimens critical to science and society.In recent years, these collections have begun accelerating the pace of digital data creation for specimen information, and crowdsourcing and citizen science have emerged as highly successful innovations that also increase public science literacy and research sustainability.

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